Nitric Oxide-Induced Polycystic Ovaries in The Wistar Rat
نویسندگان
چکیده
BACKGROUND Nitric oxide (NO) involves in polycystic ovary syndrome (PCOS), a cause of infertility in women during the reproductive age. The PCOS is now categorized as an inflammatory phenomenon. The aim of this study was to evaluate the role of NO, a proinflammatory agent, in this syndrome at histological and biochemical levels. MATERIALS AND METHODS In this experimental study, animals were female Wistar rats (weighing 200-250 g) kept under standard conditions. L-Arginine (50-200 mg/kg), a precursor of NO, was injected intra-peritoneally (i.p.) through a period ranging from 9 to14 days/ once a day. The rats' estrous cycle was studied using Papanicolaou test; those showing phase of Diestrous were grouped into experimental and control groups. The control group solely received saline (1 ml/kg, i.p.) throughout all experiments. To evaluate the inflammatory effect of NO, the rats were treated an anti-inflammatory agent, naloxone hydrochloride (0.4 mg/kg, i.p.), prior to L-arginine. At the end of the treatment period all animals' ovaries were assessed for histopathological and histochemical investigations. Also, activation of NO synthase (NOS) in the experiments was studied using NADPH-diaphorase technique. RESULTS The ovaries of rats treated with L-arginine showed polycystic characteristics in contrast to those collected from control or naloxone pretreated groups, based on image analysis. A difference in enzyme activation was also shown in the sections that belonged to the groups that received L-arginine when compared with the pre-naloxone and control groups. CONCLUSION Based on these results, we believe that NO may play a major role in the pathophysiology of PCOS.
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P-67: Nitric Oxide Has Vigor on Polycystic Ovarian Syndrome (PCOS
Background: One of the main causes of infertility in women through their breeding ages is known as polycystic ovary syndrome (PCOS). The involvement of nitric oxide (NO) in the syndrome has been shown. In this research we sought the role of NO in the PCOS by activating of the enzyme responsible for the NO producing using L-arginine, a precursor of NO. Materials and Methods: Animals were female ...
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